RMT union members are to strike over the dismissal over a fellow staff member.
Following a ballot for strike action and action short of a strike, in a bid to make First Great Western management "see sense" over the dismissal over a colleague.
Conductors, guards and train drivers at Penzance are set to strike from 4am on Friday, March 27 until 3.59am on Saturday, March 28 and from 6am on Sunday, April 5 until 5.59am on Tuesday, April 7.
Union members will also refuse to work any overtime from 4am on Friday, March 27 until further notice.
RMT General Secretary Mick Cash said: "RMT will not allow this culture to go unchallenged and the union will defend both our individual members and the principle of workplace justice.
"The union remains available for talks."
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